Selected publication milestones, not an exhaustive history or discovery priority claim.
2006 — 2006Drosophila genome-wide lamina mapping establishes the descriptive low-expression association. Original PMID16878134.
2008 — 2008synthetic peripheral transgene targeting retains inducible transcriptional competence. Original PMID18195101.
2011 — 2011native lamin-null stem cells reveal tissue-building versus global-silencing distinctions. Original PMID22116031.
2012 — 2012local HDAC/lamin perturbations separate repression and position inDrosophila. Original PMID23226217.
2013 — 2013LBRandA-type lamin tethers show sequential redundancy and opposite developmental effects. Original PMID23374351.
2018 — 2018triple-lamin knockout/add-back tests topology while detached LAD genes resist uniform derepression. Original PMID30201095.
2024 — 2024LADgenetic screens reveal local chromatin-regulator and promoter-context dependence. Original PMID39322756.
2026 — 2026LBR/LAP2deconvolution separates short detachment from chronic transcription/homeostasis effects. Original PMID41735607.
2026 — 2026B-lamin knockout maps speckle-distance andRNAchanges with cell-viability confounding retained. Original PMID41909953.
Separate necessity of a silencing enzyme from necessity of its recruitment by lamin.
Quantify native transcription after rapid detachment before chronic epigenetic/stress amplification.
Dissect B-type versus A-type lamin functions and LBR/LAP2/LEM/PRR14 redundancy by cell state.
Test whether speckle distance mediates RNA changes rather than being a correlated consequence of global disorganization.
Separate mechanical and chromatin-tether rescue with endogenous matched alleles.
Retain active LAD promoters and opposing differentiation effects in global prediction tests.
Engineer an endogenous lamin allele that loses a specified silencer interaction while preserving filament mechanics, envelope integrity and redundant-tether localization. Compare it with a mechanical-defect allele and expression-matched rescue across predefined stem and differentiated states. Perform short-time-course native nascent RNA plus contact/speckle mapping, with cell-cycle, replication stress and viability controls. Re-tether the enzyme selectively at native affected loci to test whether repression is restored before downstream damage.